Hepatocarcinoma Discrimination by Ratiometric Lipid Profiles Using Tip-Contact Sampling/Ionization Mass Spectrometry

Discrimination of human astrocytoma subtypes by lipid analysis using desorption electrospray ionization imaging mass spectrometry.

Tobacco Smoke-Induced Alterations in Hepatic Lipid Profiles Demonstrated by Imaging Mass Spectrometry

To account for differences in susceptibility to alcoholic liver disease (ALD), tobacco smoking should be evaluated as a potential cofactor given the very high percentage of heavy drinkers also smoke, and the NNK tobacco-specific nitrosamine was shown to cause steatohepatitis and exacerbate molecular and biochemical effects of alcohol on the liver. Since one of the key factors linked to ALD prog...

Analysis of eicosanoids and related lipid mediators using mass spectrometry.

Eicosanoids are oxidized arachidonate-derived lipids products generated by lipoxygenase, cyclo-oxygenase and cytochrome P450. Their bioactivity is mediated via receptor-dependent mechanisms and they are involved in a diverse array of processes in both health and disease. For many years, GC-MS was the method of choice for eicosanoid analysis, but, more recently, the availability of high-sensitiv...

Classifying human brain tumors by lipid imaging with mass spectrometry.

Brain tissue biopsies are required to histologically diagnose brain tumors, but current approaches are limited by tissue characterization at the time of surgery. Emerging technologies such as mass spectrometry imaging can enable a rapid direct analysis of cancerous tissue based on molecular composition. Here, we illustrate how gliomas can be rapidly classified by desorption electrospray ionizat...

Visualization of lipid droplet composition by direct organelle mass spectrometry.

An expanding appreciation for the varied functions of neutral lipids in cellular organisms relies on a more detailed understanding of the mechanisms of lipid production and packaging into cytosolic lipid droplets (LDs). Conventional lipid profiling procedures involve the analysis of tissue extracts and consequently lack cellular or subcellular resolution. Here, we report an approach that combin...